Effect of Ce3+ on membrane permeability of Escherichia coli cell
来源期刊：JOURNAL OF RARE EARTHS2012年第9期
论文作者：陈爱美 施庆珊 欧阳友生 陈仪本
文章页码：947 - 951
摘 要：This study aimed to delineate the antibacterial mechanism of rare-earth ion Ce 3+ to the target organism Escherichia coli cell, and the most important purpose was to identify its biological effect of increasing the E. coli cell membrane permeability. The antibacterial activities of Ce 3+ to E. coli cells were tested, and then the permeability of outer membrane （OM） and inner membrane （IM） were studied by N-phenyl-1-naphthylamine （NPN） and o-nitrophenyl-β-D-galactopyranoside （ONPG） methods separately. Through these experiments we concluded that the E. coli cells grown to log phage were more sensitive to Ce 3+ than the ones not at this stage; the structure of membrane was destroyed and the permeability of both OM and IM was obviously increased by Ce 3+ ; there should be certain interactions between Ce 3+ and some proteins inside the cell, which impeded the physiological activities of bacteria.
陈爱美，施庆珊，欧阳友生，陈仪本
摘 要：This study aimed to delineate the antibacterial mechanism of rare-earth ion Ce 3+ to the target organism Escherichia coli cell, and the most important purpose was to identify its biological effect of increasing the E. coli cell membrane permeability. The antibacterial activities of Ce 3+ to E. coli cells were tested, and then the permeability of outer membrane （OM） and inner membrane （IM） were studied by N-phenyl-1-naphthylamine （NPN） and o-nitrophenyl-β-D-galactopyranoside （ONPG） methods separately. Through these experiments we concluded that the E. coli cells grown to log phage were more sensitive to Ce 3+ than the ones not at this stage; the structure of membrane was destroyed and the permeability of both OM and IM was obviously increased by Ce 3+ ; there should be certain interactions between Ce 3+ and some proteins inside the cell, which impeded the physiological activities of bacteria.
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