Effect of a Nerve Graft Substitute Single-walled Carbon Nanotubes on Rat Pheochromocytoma Cells
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2015年第4期
论文作者：吴茜 ZHANG Juan 胡哲勤
文章页码：847 - 851
摘 要：Carbon nanotubes（CNTs） were extensively explored for their beneficial use in nervous system tissue engineering. However, an important concern regarding the use of CNTs is their toxicity during the interaction between cells and the nano particles. The rat pheochromocytoma cell line（PC12） was co-cultured with three types of single-walled carbon nanotubes（SWNTs）, purified raw SWNTs（C）, hydroxyl purified SWNTs（C-OH） and carboxyl purified SWNTs（C-COOH） at 25 μg/m L and 100 μg/ml. The experimental results revealed that SWNTs at the concentration below 100 μg/m L did not affect the cell viability. Notably, powerful antioxidant system in nerous system tissue is able to counteract with the toxicity of CNTs, which is characterized by the prominently enhanced expression of main antioxidant enzymes（catalase（CAT）, superoxide dismutase（SOD）, glutathione peroxidase（GPx） and glutathione-S-transferase（GST））. Therefore, we believe that CNTs can be good candidates for the fabrication of biomedical scaffolds for the nerve tissue repair.
吴茜，ZHANG Juan，胡哲勤
Maternal and Child Health Hospital of Hubei
摘 要：Carbon nanotubes（CNTs） were extensively explored for their beneficial use in nervous system tissue engineering. However, an important concern regarding the use of CNTs is their toxicity during the interaction between cells and the nano particles. The rat pheochromocytoma cell line（PC12） was co-cultured with three types of single-walled carbon nanotubes（SWNTs）, purified raw SWNTs（C）, hydroxyl purified SWNTs（C-OH） and carboxyl purified SWNTs（C-COOH） at 25 μg/m L and 100 μg/ml. The experimental results revealed that SWNTs at the concentration below 100 μg/m L did not affect the cell viability. Notably, powerful antioxidant system in nerous system tissue is able to counteract with the toxicity of CNTs, which is characterized by the prominently enhanced expression of main antioxidant enzymes（catalase（CAT）, superoxide dismutase（SOD）, glutathione peroxidase（GPx） and glutathione-S-transferase（GST））. Therefore, we believe that CNTs can be good candidates for the fabrication of biomedical scaffolds for the nerve tissue repair.
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