DNA Damage Effect of Mixed Rare Earth Changle Crossing Placenta Barrier on Rat Embryo
来源期刊：JOURNAL OF RARE EARTHS2003年第2期
论文作者：李树蕾 陈辉 周莉 聂毓秀 黄可欣
Key words：toxicology; mixed rare earths; embryo; placenta barrier; DNA damage;
Abstract: To assess the potential risk of mixed rare earths Changle for human embryo we used transplacental micronucleus test and single cell gel electrophoresis (SCGE) technique to detect DNA damage of embryo. The rats were administered respectively 0.3, 2, 5 and 20 mg*kg-1 mixed rare earths Changle every day orally from 6th to 18th day after pregnancy. The results show that the number of cells with micronucleus significantly increases as compared with the control except 0.3 mg*kg-1 group, which appears to be a dose-effect relationship. The number of comet star cell greatly increases with increasing contamination dose as compared with the control except 0.3 mg*kg-1 group, and also displays a dose-effect relationship. In conclusion, though mixed rare earth Changle is restricted by placenta membrane to enter embryo body, and more than 2 mg*kg-1 mixed rare earth Changle may cross placenta barrier and cause DNA damage of hepatocyte and developing erythrocyte of rat embryo.
李树蕾1，陈辉2，周莉2，聂毓秀2，黄可欣2
(1.Jilin Health School, Jilin 132011, China;
2.Department of Histology and Embryology School of Basic Medical Sciences, Jilin University, Changchun 130021, China)
Abstract:To assess the potential risk of mixed rare earths Changle for human embryo we used transplacental micronucleus test and single cell gel electrophoresis (SCGE) technique to detect DNA damage of embryo. The rats were administered respectively 0.3, 2, 5 and 20 mg*kg-1 mixed rare earths Changle every day orally from 6th to 18th day after pregnancy. The results show that the number of cells with micronucleus significantly increases as compared with the control except 0.3 mg*kg-1 group, which appears to be a dose-effect relationship. The number of comet star cell greatly increases with increasing contamination dose as compared with the control except 0.3 mg*kg-1 group, and also displays a dose-effect relationship. In conclusion, though mixed rare earth Changle is restricted by placenta membrane to enter embryo body, and more than 2 mg*kg-1 mixed rare earth Changle may cross placenta barrier and cause DNA damage of hepatocyte and developing erythrocyte of rat embryo.
Key words:toxicology; mixed rare earths; embryo; placenta barrier; DNA damage;
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