One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay
来源期刊：JOURNAL OF RARE EARTHS2021年第1期
论文作者：Zhenhua Li Qingyun Liu Yongfang Li Wei Yuan Fuyou Y.Li
摘 要：Lateral flow immunoassays（LFIAs） provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment follow-up.Lanthanide-based LFIAs is one of the most widely used methods,especially Eu（Ⅲ） chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes shift.Herein,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere（OS-EuCM）,which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling method.Benefiting from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL This lanthanide-based microsphere holds great potential for rapid pointof-care screening and clinical application.
Zhenhua Li，Qingyun Liu，Yongfang Li，Wei Yuan，Fuyou Y.Li
State Key Laboratory of Molecular Engineering of Polymers,Department of Chemistry,Fudan University
摘 要：Lateral flow immunoassays（LFIAs） provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment follow-up.Lanthanide-based LFIAs is one of the most widely used methods,especially Eu（Ⅲ） chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes shift.Herein,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere（OS-EuCM）,which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling method.Benefiting from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL This lanthanide-based microsphere holds great potential for rapid pointof-care screening and clinical application.
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