Modifi cation of β-TCP/PLGA Scaffold and Its Effect on Bone Regeneration in vivo
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2016年第2期
论文作者：林柳兰 GAO Haitao
文章页码：454 - 460
摘 要：In order to look for the best proportion of β-tricalcium phosphate（β-TCP）and poly（lactideco-glycolide）（PLGA）we fabricated porous composites β-TCP/PLGA scaffold using freeze-drying method.Morphologicalcharacterization using scanning electron microscopy showed that the interconnected pore distribution was even and there was no significant difference with the increase of PLGA content.Moreover,the porosity,compressive strength and degradation in vitro were characterized.The fabricated scaffolds with increased PLGA in the composites β-TCP/PLGA scaffolds willget stronger mechanicalproperty and better appearance,furthermore,get suitable environment for cells.According to the evaluation indexes for the tissue engineering scaffold,the group of scaffold（β-TCP/PLGA=6:4）was selected to evaluate the induced celladhesion and proliferative ability of the scaffolds.Then as transplant embed into the bone criticaldefect sites on rats femur.The repairing processes of bone defect sites were characterized by X-ray analysis within 12 weeks.X-ray analysis showed that the bone defect sites alldisplayed the formation of callus obviously,In summary,our data suggest that the scaffold（β-TCP/PLGA=6:4）has a promising clinicalfuture in regeneration of bone criticaldefects.
林柳兰1,2，GAO Haitao1
1. School of Mechatronics Engineering and Automation,Shanghai University2. School of Medicine,Shanghai Jiao Tong University
摘 要：In order to look for the best proportion of β-tricalcium phosphate（β-TCP）and poly（lactideco-glycolide）（PLGA）we fabricated porous composites β-TCP/PLGA scaffold using freeze-drying method.Morphologicalcharacterization using scanning electron microscopy showed that the interconnected pore distribution was even and there was no significant difference with the increase of PLGA content.Moreover,the porosity,compressive strength and degradation in vitro were characterized.The fabricated scaffolds with increased PLGA in the composites β-TCP/PLGA scaffolds willget stronger mechanicalproperty and better appearance,furthermore,get suitable environment for cells.According to the evaluation indexes for the tissue engineering scaffold,the group of scaffold（β-TCP/PLGA=6:4）was selected to evaluate the induced celladhesion and proliferative ability of the scaffolds.Then as transplant embed into the bone criticaldefect sites on rats femur.The repairing processes of bone defect sites were characterized by X-ray analysis within 12 weeks.X-ray analysis showed that the bone defect sites alldisplayed the formation of callus obviously,In summary,our data suggest that the scaffold（β-TCP/PLGA=6:4）has a promising clinicalfuture in regeneration of bone criticaldefects.
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