Biocompatibility evaluation in vitro. Part I: Morphology expression and proliferation of human and rat osteoblasts on the biomaterials
来源期刊:中南大学学报(英文版)2001年第1期
论文作者:RUAN Jian-ming GRANT M. Helen
文章页码:1 - 8
Key words:biomaterials; tissue culture; cell morphology; proliferation
Abstract: The biocompatibility evaluation of calcium phosphate based biomaterials is performed by tissue culture in vitro model. Three kinds of bioceramic materials which are potential to deal with bone trauma and/or conduct tissue growth are recommodated. The biological research results show that human and animal osteoblast cells anchorthe materials surface in two hours in culture. Confocal laser scanningmicroscopy (CLSM) demonstrated the normal cell distribution and proliferation on both of dense and porous biomaterials. Hydroxya-patite and tricalcium phosphate stimulate cell proliferation. However, DNA and protein synthesiswere consid-erably limited and the apoptosis phenomenonwould be presenton the hydroxyapatite (HA) materials by adding Al, Mg elements. Several important methods of biocompatibility evaluation of implant materials are described and the related biological molecular techniques such as tissue culture, cell transfection, cellular DNA stain,and Lowry assay are involved in the present research.