Effect of Mineralized Layer Topographies on Stem Cell Behavior in Microsphere Scaffold
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2016年第9期
论文作者：Jie Hou Huichang Gao Yingjun Wang Delin Cheng Xiaodong Cao
文章页码：971 - 977
摘 要：Modifying substrates through mineralization is a popular way to improve the osteogenic performance.Screening of the best mineralization characteristics on specific substrates for stem cells is meaningful but not fully studied. In this paper, poly（lactic-co-glycolic acid）/hydroxyapatite（PLGA/HA, PH） microsphere scaffolds with superficial pores were fabricated by a low-temperature fusion method. After the mineralization in the 5× stimulated body fluid（SBF） for 0, 7, 12 and 24 h, four mineralized scaffolds（MPH-0,MPH-7, MPH-12 and MPH-24） with different apatite topographies were obtained. It was found that the surface of MPH-7 was evenly decorated with abundant micro-pores, MPH-12 with dense and plain apatite layer, and MPH-24 with small spherical bumps. The responses of mouse bone mesenchymal stem cells（m BMSCs） to the four scaffolds were further studied. The results showed that MPH-7 and MPH-24 had more obvious effects on m BMSCs attachment, proliferation and differentiation than MPH-0 and MPH-12. This work indicated that to obtain the maximum improvement, the mineralization characteristics had to be carefully chosen. This was noteworthy in the chemical modification of surfaces to form the functionalized scaffolds for bone repair.
Jie Hou1,2,3，Huichang Gao1,2，Yingjun Wang1,2,3，Delin Cheng4，Xiaodong Cao1,2,3
1. School of Materials Science and Engineering, South China University of Technology2. National Engineering Research Center for Tissue Restoration and Reconstruction3. Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology4. Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
摘 要：Modifying substrates through mineralization is a popular way to improve the osteogenic performance.Screening of the best mineralization characteristics on specific substrates for stem cells is meaningful but not fully studied. In this paper, poly（lactic-co-glycolic acid）/hydroxyapatite（PLGA/HA, PH） microsphere scaffolds with superficial pores were fabricated by a low-temperature fusion method. After the mineralization in the 5× stimulated body fluid（SBF） for 0, 7, 12 and 24 h, four mineralized scaffolds（MPH-0,MPH-7, MPH-12 and MPH-24） with different apatite topographies were obtained. It was found that the surface of MPH-7 was evenly decorated with abundant micro-pores, MPH-12 with dense and plain apatite layer, and MPH-24 with small spherical bumps. The responses of mouse bone mesenchymal stem cells（m BMSCs） to the four scaffolds were further studied. The results showed that MPH-7 and MPH-24 had more obvious effects on m BMSCs attachment, proliferation and differentiation than MPH-0 and MPH-12. This work indicated that to obtain the maximum improvement, the mineralization characteristics had to be carefully chosen. This was noteworthy in the chemical modification of surfaces to form the functionalized scaffolds for bone repair.
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