Effects of Er3+ on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro
来源期刊：JOURNAL OF RARE EARTHS2011年第5期
论文作者：张金超 孙静 张大威 李亚平 郝晓红 秦新英
文章页码：507 - 510
摘 要：A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1×10-7 mol/L,but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1×10-8,1×10-7,and 1×10-6 mol/L,but had no effect at a higher concentration of 1×10-5 mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1×10-7,1×10-6,and 1×10-5 mol/L,but had no effect at a lower concentration of 1×10-8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10-6 mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.
张金超1，孙静2，张大威3，李亚平1，郝晓红1，秦新英1
1. Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University2. Affiliated Hospital of Hebei University3. Department of Natural Product Chemistry,Shenyang Pharmaceutical University
摘 要：A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1×10-7 mol/L,but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1×10-8,1×10-7,and 1×10-6 mol/L,but had no effect at a higher concentration of 1×10-5 mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1×10-7,1×10-6,and 1×10-5 mol/L,but had no effect at a lower concentration of 1×10-8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10-6 mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.
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