Lutein and lutein esters in marigold flowers by high performance chromatography
来源期刊：中南大学学报(英文版)2005年第3期
论文作者：蒋新宇 陈龙胜 周春山
文章页码：306 - 308
Key words：HPLC; lutein; lutein ester; marigold flower
Abstract: Lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography (HPLC) with ODS-C18column.A mixture of CH3CN CH3OH CH3COOCH2CH3 with volume ratio of 55∶1∶44 was used as mobile phase at a flow rate of 1.0 mL/min and detection was carried out at 460 nm. The column temperature was about 20℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications.
