Effect of Cerium on Activity of α-Amylase from Porcine Pancreas
来源期刊：JOURNAL OF RARE EARTHS2002年第5期
论文作者：苏国兴 洪法水 潘兴法 沈颂东 王雪峰
Key words：rare earths; cerium; α-amylase; activation;
Abstract: The activity of α-amylase from porcine pancreas was enhanced under the treatment by Ce3+ of low concentration (0.5～10 μmol*L-1), but was inhibited by Ce3+ of high concentration (>10 μmol*L-1). Ce3+ at high concentration displaced Ca2+ from α-amylase competitively. The equilibrium dialysis demonstrates that α-amylase has five Ca2+-binding sites with different affinities. The fluorescence titration shows that Ce3+ can bind to Ca2+-binding sites.
苏国兴1，洪法水1，潘兴法1，沈颂东1，王雪峰1
(1.Department of Biology, Life Sciences College, Suzhou University, Suzhou 215151 , China)
Abstract:The activity of α-amylase from porcine pancreas was enhanced under the treatment by Ce3+ of low concentration (0.5～10 μmol*L-1), but was inhibited by Ce3+ of high concentration (>10 μmol*L-1). Ce3+ at high concentration displaced Ca2+ from α-amylase competitively. The equilibrium dialysis demonstrates that α-amylase has five Ca2+-binding sites with different affinities. The fluorescence titration shows that Ce3+ can bind to Ca2+-binding sites.
Key words:rare earths; cerium; α-amylase; activation;
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