Amperometric detection of gold by differential pulse voltammetry using a DNA biosensor
来源期刊：Rare Metals2007年第2期
论文作者：GAN Ning, WANG Zhiying, XU Weiming, and PAN Jianguo The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ninbo University, Ningbo , China
文章页码：169 - 175
摘 要：A DNA biosensor with [Ru（DA-bpy）3]Cl2（DA-bpy:4,4’-diamino-2,2’-bipyridine） （RuL） as the electrochemical probe was prepared on pyrolytic graphite electrode （PGE） through the supramolecular interaction between RuL complex and DNA template. Cyclic voltammetry of RuL-DNA film showed a pair of stable and reversible peaks corresponding to the Ru（III）/Ru（II） redox potential of -0.165 V versus Ag|AgCl in pH 7.4 0.1 mol·L-1 Tris-HCl. The electron transfer was expected across the double-strand DNA by an "electron tunneling" mechanism. When the DNA biosensor was immerged in gold （III） buffer solution, the current peak signal （I） of the RuL-DNA supramolecular depressed and △I was linear in the concentration range of Au ion from 1 × 10-7 to 2 × 10-5 mol·L-1 with a regression coefficient of 0.9879. The detection limit was 5 × 10-8 mol·L-1. The developed procedures were applied to the analysis of synthetic samples of real materials with good sensitivity and selectivity.
GAN Ning, WANG Zhiying, XU Weiming, and PAN Jianguo The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ninbo University, Ningbo 315211, China
摘 要：A DNA biosensor with [Ru（DA-bpy）3]Cl2（DA-bpy:4,4’-diamino-2,2’-bipyridine） （RuL） as the electrochemical probe was prepared on pyrolytic graphite electrode （PGE） through the supramolecular interaction between RuL complex and DNA template. Cyclic voltammetry of RuL-DNA film showed a pair of stable and reversible peaks corresponding to the Ru（III）/Ru（II） redox potential of -0.165 V versus Ag|AgCl in pH 7.4 0.1 mol·L-1 Tris-HCl. The electron transfer was expected across the double-strand DNA by an "electron tunneling" mechanism. When the DNA biosensor was immerged in gold （III） buffer solution, the current peak signal （I） of the RuL-DNA supramolecular depressed and △I was linear in the concentration range of Au ion from 1 × 10-7 to 2 × 10-5 mol·L-1 with a regression coefficient of 0.9879. The detection limit was 5 × 10-8 mol·L-1. The developed procedures were applied to the analysis of synthetic samples of real materials with good sensitivity and selectivity.
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