Extraction and Characterization of PHB from Acidiphilium cryptum DX1-1
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2010年第6期
论文作者：徐爱玲 夏金兰
文章页码：938 - 943
摘 要：A new bacterial strain,was designated as strain Acidiphilium cryptum DX1-1,accumulates intracellular poly-β-hydroxybutyrate particles,four methods which have advantages and disadvantages for each were employed to extract PHB.Chloroform-sodium hypochlorite method is the best in extracting PHB form Acidiphilium cryptum DX1-1.The extraction rate reaches 73%,the purification rate is 92% and molecular weight is 326 kg/mol.Then the PHB extracted by this method was analyzed by ultraviolet-visible absorption spectroscopy,fourier transform infrared （FT-IR） and nuclear magnetic resonance （NMR）.The results show that PHB from strain DX1-1 has the same biochemical structure and character with PHB standard.Mass spectrometer （MS） analysis reveals that the long chain of PHB is destroyed when treated by chloroform-sodium hypochlorite.The differential scanning calorimetry （DSC） of PHB shows PHB from Acidiphilium cryptum DX1-1 has low degree of crystallinity which makes the PHB has a wider range of applications.
徐爱玲，夏金兰
Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University
摘 要：A new bacterial strain,was designated as strain Acidiphilium cryptum DX1-1,accumulates intracellular poly-β-hydroxybutyrate particles,four methods which have advantages and disadvantages for each were employed to extract PHB.Chloroform-sodium hypochlorite method is the best in extracting PHB form Acidiphilium cryptum DX1-1.The extraction rate reaches 73%,the purification rate is 92% and molecular weight is 326 kg/mol.Then the PHB extracted by this method was analyzed by ultraviolet-visible absorption spectroscopy,fourier transform infrared （FT-IR） and nuclear magnetic resonance （NMR）.The results show that PHB from strain DX1-1 has the same biochemical structure and character with PHB standard.Mass spectrometer （MS） analysis reveals that the long chain of PHB is destroyed when treated by chloroform-sodium hypochlorite.The differential scanning calorimetry （DSC） of PHB shows PHB from Acidiphilium cryptum DX1-1 has low degree of crystallinity which makes the PHB has a wider range of applications.
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