Determination of paclitaxel in southern yew tree by HPLC

来源期刊：中南大学学报(英文版)2003年第3期

论文作者：施树云 周春山

文章页码：207 - 210

Key words：high performance liquid chromatography; paclitaxel; southern yew tree

Abstract: Paclitaxel in southern yew tree was quantitatively determined by high performance liquid chromatography (HPLC) with ODS-C₁₈ column. A mixture of CH₃OH-H₂O-CH₃COOH(volume ratio: 55:44:1) is used as mobile phase and UV detectioH is carried out at 227 nm, and the column temperature is 200C.The results show that there is a good linear relationship between the area of paclitaxel and the concentration of the sample in the range 50-500 mg/L for paclitaxel. The corresponding regression equation is Y=13 021. 7+1. 01×0⁶ X, r=0. 999 0. The average recovery is 95. :3 % and the relative standard deviation is 2. 08 0 0.